The PLP is anchored by means of a lysine, which forms an aldimine Schiff base linkage with the PLP aldehyde.
22.
For example, Schiff base formation can be categorized as a forming new covalent bonds between a carbonyl and primary amine.
23.
Retinal binds covalently to a lysine on the transmembrane helix nearest the C-terminus of the protein through a Schiff base linkage.
24.
If this group is " not " a hydrogen atom, then the compound can sometimes be referred to as a Schiff base.
25.
The second water molecule is a product of the dehydration of the carbinolamine that leads to the formation of the Schiff base.
26.
The Mannich reaction is an example of nucleophilic addition of an amine to a carbonyl group followed by dehydration to the Schiff base.
27.
The reaction mechanism proceeds via the formation of a Schiff base intermediate, which is covalently attached to lysine 115 in the active site.
28.
The Lysine, which is involved in the formation of the Schiff base is coordinated by a phosphate ion and two solvent water molecules.
29.
The term Schiff base is normally applied to these compounds when they are being used as ligands to form coordination complexes with metal ions.
30.
These results provided support that the mechanism proceeds through a Schiff base intermediate between the ketoacid and an amino acid residue on the enzyme.
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