In karyotype analysis, a silver stain can be used to identify the NOR . Silver nitrate inserts into the NOR-associated protein in the stalks and satellites, staining the proteins dark black.
22.
Short of staining your entire 2D gel for protein ( silver stain, Coomassie, Sypro orange, etc . ) and cutting out every spot for analysis, I can't think of any reliable way to find a non-radioactive target.
23.
Silver stain is a common problem in roller transporter processors, since the material ( usually paper ) is pinched between squeegee rollers to remove excess developer and brought into the fixing bath without intermediate rinse or stop.
24.
For example, a time course analysis of neuronal degeneration between the two techniques shows that silver stain is evident earlier after a neurotoxic insult which may suggest that fluoro-jade is specific for a later, more committed stage of the degenerative process.
25.
Although most of the original stained glass at Mantes has been lost ( mostly during an outbreak of Revolutionary iconoclasm in 1794 ), the south side of the Navarre Chapel retains four excellent early 14th century roundels depicting Passion scenes, which show a restrained early use of silver stain.
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