The protoplast are fist culture in liquid medium at 25 to 28 C with a light intensity of 100 to 500 lux or in dark and after undergoing substantial cell division, they are transferred into solid medium congenial or morphogenesis in many horticultural crops response well to protoplast culture.
22.
When grown on solid medium AOM1, colonies were light-pink pigmented, circular, convex, relatively small ( 2 4 mm ) in size after one week of incubation at 60 �C . " A . rosea " has an optimum temperature at 68 �C with a range of 50 73 �C . The optimum pH for growth was 5.3 with a range of 4.5 5.8.
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