21. In mammalian cell culture, the analogous process of introducing DNA into cells is commonly termed transfection . 22. Internal controls are also necessary to normalise for differences in transfection efficiencies and gene expression in different cells. 23. Because of this, electroporation is one of the key methods of transfection as well as bacterial transformation. 24. Poly ( ethylenimine ) was the second polymeric transfection agent discovered, after poly-l-lysine. 25. Another application is the use of GFP co-transfection as internal control for transfection efficiency in mammalian cells. 26. Another application is the use of GFP co-transfection as internal control for transfection efficiency in mammalian cells. 27. Raji cell is widely used as a transfection host and also to understand the hematopoietic and other cell malignancies. 28. By transfection of endothelial cells through the use of lipoplexes, physical alterations in the cells could be made. 29. Transfection of breast cancer cells with ferroportin significantly reduces their growth after orthotopic implantation in the mouse mammary fat pad.30. Transfection of different cell types with wild-type p53 was associated with a strong repression of the survivin promoter.
