Next, multiple label probe oligonucleotides ( conjugated to alkaline phosphatase or directly to fluorophores ) hybridize to each amplifier molecule.
32.
Shrimp alkaline phosphatase ( SAP ) or Antarctic phosphatase ( AP ) are suitable alternative as they can be easily inactivated.
33.
However, this study revealed that without the influence of sodium butyrate, alkaline phosphatase activity is fairly low in cancer cells.
34.
This explains why the addition of sodium butyrate show increased activity of alkaline phosphatase in the cancer cells of the human colon.
35.
Engvall and Perlmann published their first paper on ELISA in 1971 and demonstrated its quantitative value using alkaline phosphatase as the reporter.
36.
Biochemical studies reveal hypophosphatemia ( low blood phosphate ), elevated alkaline phosphatase and low serum 1, 25 dihydroxyvitamin D levels.
37.
Studies show that the alkaline phosphatase protein found in cancer cells has similar characteristics to that found in non-malignant body tissues.
38.
As the osteoblast transitions to an osteocyte, alkaline phosphatase is reduced, and casein kinase II is elevated, as is osteocalcin.
39.
Tissue non-specific alkaline phosphatase ( TNSALP ) deficiency in osteoblasts and chondrocytes impairs bone mineralization, leading to rickets or osteomalacia.
40.
NPP s promiscuity enables the enzyme to share substrates with alkaline phosphatase ( AP ), another member of the alkaline phosphate superfamily.
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