31. Because of the close proximity of the 5'tail it anneals to the 3'end of the primer. 32. Distinctively, though, he anneals his cutting-edge irony into a compassionate anger that goes beyond the literary times. 33. The temperature required to anneal a particular steel depends on the type of annealing to be achieved and the alloying constituents. 34. After mixing and denaturation, the strands are allowed to anneal to produce three different combinations as detailed in the diagram. 35. Primers should also not anneal strongly to themselves, as internal hairpins and loops could hinder the annealing with the template DNA. 36. Once off the bath, the glass sheet passes through a anneals without strain and does not crack from the temperature change. 37. The primer will anneal at the highest temperature which is least-permissive of nonspecific binding that it is able to tolerate. 38. The AAV ITRs of two genomes can anneal to form head to tail concatamers, almost doubling the capacity of the vector. 39. The duplex is denatured and the second primer anneals to the newly formed DNA strand, containing sequence from the first primer. 40. If the probe sequence of the molecular beacon encounters its target genomic DNA during the assay, it will anneal and hybridize.
