31. Complete inactivation of avidin's biotin binding capacity required boiling for over 4 minutes. 32. For visualization purposes, the nucleic acid fragment is usually labelled with a biotin label. 33. The enzyme biotinidase cleaves biocytin and makes biotin available to be reused by other enzymes. 34. Biotin is commonly found in multivitamin supplements.35. Concerning organic requirements, most strains of " S . cerevisiae " require biotin . 36. Biotin deficiency can result in behavioral disorders, lack of coordination, learning disabilities and seizures.37. The symptoms of biotinidase deficiency ( and dietary deficiency of biotin ) can be quite severe. 38. Raw avidin immobilises biotin , so excessive feeding of raw eggs may cause fatal biotin deficiency. 39. Raw avidin immobilises biotin, so excessive feeding of raw eggs may cause fatal biotin deficiency. 40. The enzyme is biotin -dependent.
