There will be both diffusion and binding, and indeed the diffusion constant may not be uniform in space, and there may be more than one type of binding sites, and these sites may also have a non-uniform distribution in space.
32.
This more complex behavior implies that a model with several parameters is required to describe the data; models with only either a single diffusion constant " D " or a single off rate constant, " k off ", are inadequate.
33.
In other words, that diffusion is occurring in a uniform medium so the effective diffusion constant " D " is the same everywhere, and that the diffusion is isotropic, i . e ., occurs at the same rate along all axes in the plane.
34.
A is the diffusion constant of the ion A . The second term reflects the flux due to the electric field, which increases linearly with the electric field; this is a faraday " F ", which is the total charge of a mole of electrons.
35.
It is defined by E _ T = \ hbar D / L ^ 2, where D is the diffusion constant and L the size of the system, and thereby inversely proportional to the diffusion time t _ D = L ^ 2 / D through the system.
36.
There are models with both diffusion and reaction . have shown that FRAP curves can be fitted by " different " pairs of values of the diffusion constant and the on-rate constant, or, in other words, that fits to the FRAP are not unique.
37.
Which connects the diffusion constant " D " and the particle mobility " ? ", the ratio of the particle's terminal drift velocity to an applied force . " k " " B " is the Boltzmann constant, and " T " is the absolute temperature.
38.
This technique is limited to particles large enough for optical detection ( in the micrometer range ), but it has the advantage of being time-resolved so that, aside from the statical information, it also gives access to dynamical parameters ( e . g . diffusion constants ) and also space-resolved ( to the level of the individual particle ), allowing it to reveal the morphology and dynamics of local structures in colloidal crystals, glasses, gels, and hydrodynamic interactions.
39.
Dynamic properties such as duplex single strand equilibria and binding rates of other molecules to duplexes can also be determined by its effect on the spin lattice relaxation time " T " 1, but these methods are insensitive to intermediate rates of 10 4 10 8 s " 1, which must be investigated with other methods such as solid-state NMR . Dynamics of mechanical properties of a nucleic acid double helix such as bending and twisting can also be studied using NMR . Pulsed field gradient NMR experiments can be used to measure diffusion constants.
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