CRISPR identification in raw reads has been achieved using purely " de novo " identification or by using direct repeat sequences in partially assembled CRISPR arrays from contigs ( overlapping DNA segments that together represent a consensus region of DNA ) as a hook for identifying direct repeats in individual reads.
32.
CRISPR identification in raw reads has been achieved using purely " de novo " identification or by using direct repeat sequences in partially assembled CRISPR arrays from contigs ( overlapping DNA segments that together represent a consensus region of DNA ) as a hook for identifying direct repeats in individual reads.
33.
The overall organisation common to all three and consists of a unique central region flanked by a direct repeat at each end . 137, 150, and 155 unique, functional protein coding genes are present in the unique regions respectively : of these six, four, and eight respectively are duplicated in the terminal repeat.
34.
The crRNA processing and interference stages occur differently in each of the three major CRISPR systems . ( 4 ) The primary CRISPR transcript is cleaved by cas genes to produce crRNAs . ( 5 ) In type I systems Cas6e / Cas6f cleave at the junction of ssRNA and dsRNA formed by hairpin loops in the direct repeat.
35.
Crystal structure of human liver X receptor ? ( LXR? ) forming heterodimer with its partner retinoid X receptor ? ( RXR? ) on its cognate element, an AGGTCA direct repeat spaced by 4 nt shows an extended X-shaped arrangement, with DNA-and ligand-binding domains crossed, in contrast to the parallel domain arrangement of other NRs that bind an AGGTCA direct repeat spaced by 1 nt.
36.
Crystal structure of human liver X receptor ? ( LXR? ) forming heterodimer with its partner retinoid X receptor ? ( RXR? ) on its cognate element, an AGGTCA direct repeat spaced by 4 nt shows an extended X-shaped arrangement, with DNA-and ligand-binding domains crossed, in contrast to the parallel domain arrangement of other NRs that bind an AGGTCA direct repeat spaced by 1 nt.
37.
The stages of CRISPR immunity for each of the three major types of adaptive immunity . ( 1 ) Acquisition begins by recognition of invading DNA by Cas1 and Cas2 and cleavage of a protospacer . ( 2 ) The protospacer is ligated to the direct repeat adjacent to the leader sequence and ( 3 ) single strand extension repairs the CRISPR and duplicates the direct repeat.
38.
The stages of CRISPR immunity for each of the three major types of adaptive immunity . ( 1 ) Acquisition begins by recognition of invading DNA by Cas1 and Cas2 and cleavage of a protospacer . ( 2 ) The protospacer is ligated to the direct repeat adjacent to the leader sequence and ( 3 ) single strand extension repairs the CRISPR and duplicates the direct repeat.
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