DMSO in a PCR reaction is applicable for supercoiled plasmids ( to relax before amplification ) or DNA templates with high GC-content ( to decrease thermostability ).
32.
When RNA polymerase II reaches a " termination sequence " ( TTATTT on the DNA template and AAUAAA on the primary transcript ), the end of transcription is signaled.
33.
During DNA replication, the replisome will unwind the parental duplex DNA into a two single-stranded DNA template replication fork in a 5'to 3'direction.
34.
The same DNA molecule can be resequenced independently by creating the circular DNA template and utilizing a strand displacing enzyme that separates the newly synthesized DNA strand from the template.
35.
After the replicative helicase has unwound the parental DNA duplex, exposing two single-stranded DNA templates, replicative polymerases are needed to generate two copies of the parental genome.
36.
When a double strand DNA lesion is repaired by NHEJ there is no validating DNA template present so it may result in a novel DNA strand formation with loss of information.
37.
Ligases are particularly important in lagging strand DNA replication, as they join together the short segments of DNA produced at the replication fork into a complete copy of the DNA template.
38.
Protein-protein interaction with the clamp prevents DNA polymerase from diffusing from the DNA template, thereby ensuring that the enzyme binds the same primer / template junction and continues replication.
39.
The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen ( PCNA ) and replication factor C ( RFC ).
40.
The 17-bp transcriptional complex has an 8-bp DNA-RNA hybrid, that is, 8 base-pairs involve the RNA transcript bound to the DNA template strand.
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