Since velocity \ V \, is proportional to the applied electric field, \ E \,, the apparent electrophoretic mobility \ \ mu _ { obs } \, is define by the equation
32.
Assays are created that combine reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence microscopy to silence the T6SS gene cluster by the histone-like nucleoid structuring H-NS protein.
33.
Proteins which associate with the promoter can be identified using an electrophoretic mobility shift assay ( EMSA ), and the effects of inclusion or exclusion of the proteins with the mutagenized promoters can be assessed in the assay.
34.
He found that, under certain conditions, sickle cell hemoglobin is positively charged while normal hemoglobin is not, creating a difference in electrophoretic mobility . which by 1958 he determined to be a glutamic acid in place of a valine.
35.
Since electrophoretic mobility of ions is generally temperature-dependent ( due to both temperature-dependent ionization and solvent viscosity effects ), a non-uniform temperature profile results in variation of electrophoretic mobility across the capillary, and a loss of resolution.
36.
Since electrophoretic mobility of ions is generally temperature-dependent ( due to both temperature-dependent ionization and solvent viscosity effects ), a non-uniform temperature profile results in variation of electrophoretic mobility across the capillary, and a loss of resolution.
37.
In some very specific cases, the detection of the phosphorylation as a shift in the protein's electrophoretic mobility is possible on simple 1-dimensional SDS-PAGE gels, as it's described for instance for a transcriptional coactivator by Kovacs et al.
38.
They introduced a new terminology for this product, calling it " Form V " ( extending the nomenclature of Forms I IV used in sedimentation studies of DNA ), and then went on to demonstrate that its electrophoretic mobility was clearly different from that of the native chromosome.
39.
FANCA binds to both single-stranded ( ssDNA ) and double-stranded ( dsDNA ) DNAs; however, when tested in an electrophoretic mobility shift assay, its affinity for ssDNA is significantly higher than for dsDNA . FANCA also binds to RNA with a higher affinity than its DNA counterpart.
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