31. In reversed phase chromatography, the most polar compounds elute first with the most nonpolar compounds eluting last. 32. In reversed phase chromatography, the most polar compounds elute first with the most nonpolar compounds eluting last. 33. After washing, TEV protease is introduced to elute the bound material at the TEV protease EGTA ). 34. Bound phage are collected by virtue of the magnetic beads and may be eluted by incubation with trypsin. 35. The enriched DNA fraction is then purified, eluted from the beads and subjected to next generation sequencing. 36. The V o component represents the volume at which the larger molecules elute , which elute in the beginning. 37. The V o component represents the volume at which the larger molecules elute, which elute in the beginning. 38. The time at which a specific analyte elutes ( emerges from the column ) is called its retention time. 39. The specifically bound DNA is eluted from each individual protein-DNA complex and analyzed by polyacrylamide gel electrophoresis. 40. Though less frequent with drug-eluting stents, neointimal proliferation can still occur in DES and cause restenosis.
