Elution of the antibodies of interest is most often achieved using a low pH buffer such as glycine pH 2.8.
32.
The separate curves in the diagram represent different sample elution concentration profiles over time based on their affinity to the column resin.
33.
The actinide elution sequence is reversed from that of the cation-exchange resin column, so that the heavier actinides elute later.
34.
One major factor affecting reproducibility in proteomics experiments is the simultaneous elution of many more peptides than can be measured by mass spectrometers.
35.
In isocratic elution, peak width increases with retention time linearly according to the equation for N, the number of theoretical plates.
36.
Alternatively, if the flow is reversed, the adsorbed particles will quickly settle and the proteins can be desorbed by an elution buffer.
37.
Another method to isolate mendelevium exploits the distinct elution properties of Md 2 + from those of Es 3 + and Fm 3 +.
38.
A separation in which the mobile phase composition is changed during the separation process is described as a " gradient elution ".
39.
The gold can be removed from the carbon by using a strong solution of caustic soda and cyanide, a process known as elution.
40.
Because of the abundant separating columns, elution systems, and detectors available, chromatography has developed into the main method for ion analysis.
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