31. The most common toxic activity is the protein acting as an endonuclease , also known as an interferase. 32. RISC has a catalytic component argonaute, which is an endonuclease capable of degrading messenger RNA ( mRNA ). 33. Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ). 34. The plasmid contains a genetic sequence corresponding to the recognition site of a restriction endonuclease , such as EcoR1. 35. As with RLGS, the endonuclease component is retained in the method but it is coupled to new technologies. 36. Sickle-cell gene mutation probably arose spontaneously in different geographic areas, as suggested by restriction endonuclease analysis. 37. The endonuclease is a dimer, requiring a second step for assembly, whereas the methylase is a monomer. 38. When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA. 39. These investigators were not able to identify the ends of the Rep / Helicase / Endonuclease unit of Helitrons. 40. Class 1 uses several Cas proteins together with the CRISPR RNAs ( crRNA ) to build a functional endonuclease .