Methods incorporating chromatography generally begin with cryodepleted plasma undergoing buffer exchange via either diafiltration or buffer exchange chromatography, to prepare the plasma for following ion exchange chromatography steps.
32.
This is usually achieved by ion-exchange chromatography, with the standard process using a cation exchanger such as Dowex 50 or T EVA eluted with a solution of ammonium ?-hydroxyisobutyrate.
33.
Ion-exchange chromatography is also useful in the isolation of specific multimeric protein assemblies, allowing purification of specific complexes according to both the number and the position of charged peptide tags.
34.
The boom of Ion exchange chromatography primarily began between 1935 1950 during World War II and it was through the " Manhattan project " that applications and IC were significantly extended.
35.
It has been proposed by many studies that the majority of the toxins are released in either late log phase or early precipitation, in lieu by either gel filtration or ion-exchange chromatography.
36.
Since 1935, ion exchange chromatography rapidly manifested into one of the most heavily leveraged techniques, with its principles often being applied to majority of fields of chemistry, including distillation, adsorption, and filtration.
37.
This form of chromatography is widely used in the following applications : water purification, preconcentration of trace components, ligand-exchange chromatography, ion-exchange chromatography of proteins, high-pH anion-exchange chromatography of carbohydrates and oligosaccharides, and others.
38.
Another consideration to take into account is that, since any amine groups will have reacted with the labelling reagent, ion exchange chromatography cannot be used, and high-pressure liquid chromatography should be used instead.
39.
This form of chromatography is widely used in the following applications : water purification, preconcentration of trace components, ligand-exchange chromatography, ion-exchange chromatography of proteins, high-pH anion-exchange chromatography of carbohydrates and oligosaccharides, and others.
40.
This form of chromatography is widely used in the following applications : water purification, preconcentration of trace components, ligand-exchange chromatography, ion-exchange chromatography of proteins, high-pH anion-exchange chromatography of carbohydrates and oligosaccharides, and others.
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