Furthermore, the time used for replication between the two strands varies and may lead to asymmetric mutational pressure between leading and lagging strand.
32.
The second school of thought describes the mechanism of GC and AT skew as resulting from differential selective pressure between the leading and lagging strands.
33.
This happens at all the sites of the lagging strand, but it does not happen at the end where the last RNA primer is attached.
34.
During DNA replication of circular bacterial genomes, multiple proteins are involved in synthesizing the leading strand, and the Okazaki fragments on the lagging strand.
35.
On the lagging strand " template ", a primase " reads " the template DNA and initiates synthesis of a short complementary RNA primer.
36.
Priming enzymes, ( which are DNA-dependent RNA polymerases ), solve this problem by creating an RNA primer on the leading and lagging strands.
37.
Then, DNA ligase I binds to the PCNA, which is clamped to the nicks of the lagging strand, and catalyzes the formation of phosphodiester bonds.
38.
The preferred DNA substrate mimics an arrested DNA replication fork with unreplicated lagging strand, structurally identical to a product of recombinational repair of a stalled replication fork.
39.
There are two problems after leading and lagging strand synthesis : RNA remains in the duplex and there are nicks between each Okazaki fragment in the lagging duplex.
40.
Due to the fact that an RNA primer is required for initiation of DNA synthesis, the lagging strand is at a disadvantage in replicating the entire chromosome.
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