31. An estimated 400 tubal ligations are performed at Grady every year, she said. 32. I had tubal ligation done four years ago. 33. The menstrual condition even has a name : post-tubal-ligation syndrome. 34. Ligation of artificial telomeric sequences to convert circular plasmid into a linear piece of DNA35. Native chemical ligation can also be performed with an N-terminal selenocysteine residue. 36. Self ligation typically cuts orthodontic appointment time drastically. 37. The idea behind using the same restriction enzymes is to create ligation later on. 38. The human women have their tubal ligation reversed. 39. Tubal ligation is major surgery that is done in a hospital and requires anesthesia. 40. This enzyme participates in benzoate degradation via hydroxylation and benzoate degradation via coa ligation .