Infused, negatively charged, immune-modifying microparticles ( IMPs ), derived from polystyrene, microdiamonds, or biodegradable poly ( lactic-co-glycolic ) acid, are taken up by inflammatory monocytes, in an opsonin-independent fashion, via MARCO . This results in monocyte sequestration in the spleen through apoptotic cell clearance mechanisms.
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Phagocytosis of such cells requires specific receptors on the phagocyte that recognise either phosphatidylserine directly or opsonins bound to the phosphatidylserine or other " eat-me " signals, such as calreticulin . " Don't-eat-me " signals include CD47, which when expressed on the surface of a cell, inhibit phagocytosis of that cell, by activating SIRP-alpha receptors on the phagocyte.
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