31. South Sea pearl oysters were in limited supply in the Philippines, and Iwasaki s project initially struggled to survive. 32. On the inner side pearl oyster from Japan were used, while on the outer side Mexican shells were set. 33. A recent project to farm black pearl oysters has been successful at generating additional income for the island's people. 34. Because it did not grow in a pearl oyster it is not pearly; instead the surface is glossy like porcelain. 35. This technology, developed in Japan, involves artificially introducing beads into pearl oysters and letting the pearls develop over several years. 36. Aspein is likely responsible for directed formation of calcite in the shell of the pearl oyster " Pinctada fucata ". 37. But it takes six to eight years for a 10-millimeter pearl to form in a pearl oyster , according to Abbott. 38. The button merchant Isaac Azarael, awaiting a lost shipment of abalone shell and pearl oyster , is Katchor's lone fixed point. 39. Mitsubishi commenced pearl culture with the South Sea pearl oyster in 1916, as soon as the technology patent was commercialized. 40. The outer layer of pearls and the inside layer of pearl oyster and freshwater pearl mussel shells are made of nacre.