PCR greatly amplifies the amounts of a specific region of DNA . In the PCR process, the DNA sample is denatured into the separate individual polynucleotide strands through heating.
32.
In 1955, Marianne Grunberg-Manago and colleagues published a paper describing the enzyme polynucleotide phosphorylase, which cleaved a phosphate group from nucleotide diphosphates to catalyze their polymerization.
33.
The RNA element's function is likely analogous to an RNA structure found upstream of polynucleotide phosphorylase genes in " E . coli " and related enterobacteria.
34.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric systematic name of this enzyme class is "'polynucleotide 3'- phosphohydrolase " '.
35.
This enzyme belongs to the family of hydrolases, specifically those acting on phosphoric systematic name of this enzyme class is "'polynucleotide 5'- phosphohydrolase " '.
36.
Her identification of the space group for DNA crystals revealed to Crick that the DNA strands were antiparallel, which helped Watson and Crick decide to look for DNA models with two antiparallel polynucleotide strands.
37.
The structure of the S1 RNA-binding domain from the " Escherichia coli " polynucleotide phosphorylase has been determined using NMR methods and consists of a five-stranded antiparallel beta barrel.
38.
The leading strand is synthesized continuously and in juxtapose to the leading strand; the lagging strand is replicated through short fragments of polynucleotide ( Okazaki fragments ) in a 5'to 3'direction.
39.
More detailed comparisons with such " ab initio " quantum computations are in principle obtainable through 2D-FT NMR spectroscopy and relaxation studies of polynucleotide solutions or specifically labeled DNA, as for example with deuterium labels.
40.
Therefore, the primer and reverse transcriptase must be relocated to 3 end of viral RNA . In order to accomplish this reposition, multiple steps and various enzymes including DNA polymerase, ribonuclease H ( RNase H ) and polynucleotide unwinding are needed.
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