Back in France, he is nominated directeur de recherche at CNRS and worked at Institut Jacques Monod where he develops from 1973 to 2001 the analysis of primary transcripts ( pre-mRNA ) and of their metabolism in functional RNA within polyribosome.
32.
A micrograph of ongoing gene transcription of ribosomal RNA illustrating the growing primary transcripts . " Begin " indicates the 5'end of the DNA, where new RNA synthesis begins; " end " indicates the 3'end, where the primary transcripts are almost complete.
33.
A micrograph of ongoing gene transcription of ribosomal RNA illustrating the growing primary transcripts . " Begin " indicates the 5'end of the DNA, where new RNA synthesis begins; " end " indicates the 3'end, where the primary transcripts are almost complete.
34.
Despite being located in intronic regions of a coding gene in the direction of transcription, it has its own promoter regions and forms a ~ 3433-nt long primary transcript of miR-21 ( known as pri-miR-21 ) which is independently transcribed.
35.
It binds to Drosha, an RNase III enzyme, to form the Microprocessor complex that cleaves a primary transcript known as pri-miRNA to a characteristic stem-loop structure known as a pre-miRNA, which is then further processed to miRNA fragments by the enzyme Dicer.
36.
A microRNA molecule is synthesized as a long RNA primary transcript known as a " pri-miRNA ", which is cleaved by Drosha to produce a characteristic stem-loop structure of about 70 base pairs long, known as a " pre-miRNA ."
37.
The primary transcript is cleaved by the Drosha ribonuclease III enzyme to produce an approximately 70-nt stem-loop precursor miRNA ( pre-miRNA ), which is further cleaved by the cytoplasmic Dicer ribonuclease to generate the mature miRNA and antisense miRNA star ( miRNA * ) products.
38.
The primary transcript originally described, contained 11 exons with protein coding beginning on exon 2 and ending in exon 11 ( transcript variant 2 ), resulting in a 398 amino acid protein with two strongly predicted transmembrane domains ( TMDs ), coded in exons 2 and 7 ( isoform 2 ).
39.
RNA polymerase is often incapable of synthesizing a primary transcript if the targeted gene's promoter region contains specific methylated cytosines residues that hinder binding of transcription-activating factors and recruit other enzymes to stabilize a tightly bound nucleosome structure, excluding access to RNA polymerase and preventing the production of primary transcripts.
40.
RNA polymerase is often incapable of synthesizing a primary transcript if the targeted gene's promoter region contains specific methylated cytosines residues that hinder binding of transcription-activating factors and recruit other enzymes to stabilize a tightly bound nucleosome structure, excluding access to RNA polymerase and preventing the production of primary transcripts.
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