After ssDNA insertion into the " V . cholerae " cytoplasm, a complementary strand of DNA is formed in order to create the plasmid form of the viral genome, pCTX . pCTX can undergo DNA replication to create new ssDNA genomes and / or be incorporated into the bacterial genome as a prophage.
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The bacteriophage T12 integrates into S . pyogenes chromosome by site-specific recombination into the anticodon loop of a gene that codes for serine tRNA . The bacterial attachment site ( attB ) has a 96 base pair sequence homologous to the phage attachment site and is located at the 3 end of the tRNA gene such that the coding sequence of the tRNA gene remains intact after integration of the prophage.
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