The enzyme "'FokI "', naturally found in " Flavobacterium okeanokoites ", is a bacterial type IIS restriction endonuclease consisting of an N-terminal DNA-binding domain and a non-specific DNA cleavage domain at the C-terminal.
32.
After development of quantitative immunological methods, his lab was the first to recognize restriction endonuclease mapping analysis as a quantitative evolutionary genetic method, which led to his early use of DNA sequencing, and the then-nascent technique of PCR to obtain large DNA sets for genetic analysis of populations.
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In the pathogenic bacterium " Neisseria meningitidis " ( meningococci ), competence for transformation is a highly evolved and complex process where multiple proteins at the bacterial surface, in the membranes and in the cytoplasm interact with the incoming transforming DNA . Restriction-modification systems are abundant in the genus " Neisseria " . " N . meningitidis " has multiple type II restriction endonuclease systems.
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The commonly used notation for restriction endonucleases is of the form " " vwxyz " ", where " " vwx " " names the life form ( bacteria ) where this restriction endonuclease may be found, " " y " " names the strain ( and is optional ), and " " z " " ( in Roman numerals ) indicates different restriction endonucleases in the same life form ( bacteria ).
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Another example would be the recent report describing a heteroplasmic mtDNA molecule containing five linked missense mutations dispersed over the contiguous mtDNA CO1 and CO2 genes in Alzheimer s disease patients, however, the more recent studies using PCR, restriction endonuclease site variant assays, and phylogenic analysis proposed that the nuclear CO1 and CO2 sequences revealed that they diverged from modern human mtDNAs early in hominid evolution about 770, 000 years before and these preserved NUMTs could cause Alzheimer s disease.
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