The second part ( the dubb ) of the " lubb-dubb " ( the " second heart sound ", or S 2 ), is caused by the closure of the aortic and pulmonary valves at the end of ventricular systole.
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The echocardiographic technique to measure the regurgitant fraction is to determine the forward flow through the mitral valve ( from the left atrium to the left ventricle ) during ventricular diastole, and comparing it with the flow out of the left ventricle through the aortic valve in ventricular systole.
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