41. Acidification lowers the level of carbonate ions available for calcifying organisms to form their shells.42. Crayfish, their main food, are sensitive to acidification and accumulation of heavy metals. 43. Acidification also occurs when leaching of bases.44. Acidification of the organic phase yields rubrocyanine, which can be detected by colorimetric methods.45. It is synthesized as a catalytically inactive enzyme which is activated and autoproteolyzed upon acidification . 46. Problems range from environmental effects of fishing techniques, described above, to ocean acidification . 47. This makes the area sensitive to acidification . 48. It is produced by distillation of the polysulfane oil obtained by acidification of polysulfide salts. 49. The acidification of the oceans would kill many organisms that build shells from calcium carbonate. 50. However, ocean acidification is predicted to occur at a rate that evolution cannot match.
