41. The true ground state of the flavin cofactor in " Drosophila" 42. The BCCP domain transfers the tethered cofactor between the two remote active sites. 43. Additional hydrolysis releases ammonia and the original cofactor , completing the catalytic cycle. 44. This is the one that requires a cofactor of TPP for its function. 45. Absorption spectrum analysis illustrates that zinc is the only cofactor present in barbiturase. 46. This is a view of the GlmS ribozyme bound to its catalytic cofactor . 47. The methodology is applicable to essentially any enzyme, including cofactor dependent oxidoreductases. 48. Human variants of Coproporphyrinogen oxidase are cofactor -independent. 49. The cofactors : iron, Tetrahydrobiopterin, and Tetrahydropteridine. 50. The cofactors : NADH, NADPH, and Heme.