The task of identifying the genetic constitution essentially involves the spelling out of the DNA ( deoxyribose nucleic acid ) sequence which forms the spiral ladder-like double helix structure.
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The enzyme follows a sequential mechanism, where phosphate binds before thymidine ( or deoxyuridine, etc . ) and 2-deoxyribose 1-phosphate leaves after the nitrogenous base.
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In the presence of DNA, the benzyne diradical abstracts hydrogens from the deoxyribose sugar backbone, predominantly at the C-1, C-4 and C-5 positions.
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Thymidine phosphorylase catalyzes the reversible phosphorylation of thymidine, deoxyuridine, and their analogs ( except deoxycytidine ) to their respective bases ( thymine / uracil ) and 2-deoxyribose 1-phosphate.
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*1953-Francis Crick and James D . Watson publish " Molecular structure of nucleic acids : a structure for deoxyribose nucleic acid " describing the double helix structure of DNA.
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If the sugar is a simple ribose, the polymer is RNA ( ribonucleic acid ); if the sugar is derived from ribose as deoxyribose, the polymer is DNA ( deoxyribonucleic acid ).
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For example, deoxyribose, a component of DNA, is a modified version of ribose; chitin is composed of repeating units of N-acetyl glucosamine, a nitrogen-containing form of glucose.
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Nucleic acid types differ in the structure of the sugar in their nucleotides DNA contains 2'- deoxyribose while RNA contains ribose ( where the only difference is the presence of a hydroxyl group ).
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For example, deoxyribose ( C 5 H 7 O in DNA, C 5 H 10 O 4 when free ), the nucleotide in the top left in the image, is read as:
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In the next step, an AP endonuclease creates a nick at the 5'end of the AP site, generating a hanging deoxyribose phosphate ( dRP ) residue in place of the AP site.
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