This happens in a dry state, without extracting the monoamines from the cells during the entire procedure, a process that starts with separation of a tissue sample and ends with a thin tissue slice that can be examined in a fluorescence microscope.
42.
He and his collaborators did extensive work on the application of flow cytometry to analyze endocytic membrane traffic beginning in the early 1980s and pioneered the application of machine learning methods to high-resolution fluorescence microscope images depicting subcellular location patterns in the mid-1990s.
43.
Another advantage of FIB secondary electron imaging is the fact that the ion beam does not alter the signal from fluorescent probes used in the labelling of proteins, thus creating the opportunity to correlate FIB secondary electron images with images obtained by fluorescence microscopes.
44.
The " fluorescence microscope " refers to any microscope that uses fluorescence to generate an image, whether it is a more simple set up like an epifluorescence microscope, or a more complicated design such as a confocal microscope, which uses optical sectioning to get better resolution of the fluorescent image.
45.
Methods of analysis in these fields are also growing, often with nomenclature in the form of acronyms such as : TIRF . Most of these techniques rely on fluorescence microscopes, which use high intensity light sources, usually mercury or xenon lamps, LEDs, or lasers, to excite fluorescence in the samples under observation.
46.
The SMI microscope being developed at the Kirchhoff Institute for Physics at Heidelberg University achieves this in the following manner : The illumination intensity within the object range is not uniform, unlike conventional wide field fluorescence microscopes, but is spatially modulated in a precise manner by the use of two opposing interfering laser beams along the axis.
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