Once the fluorophore is distantiated from the quencher, illumination of the hybrid with light results in the fluorescent emission.
42.
Based on crystallographic data, massive rearrangement of the adjacent bases occurs once the fluorophore binds to accommodate the molecule.
43.
Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein or rhodamine ( see immunofluorescence ).
44.
If the fluorophore absorbs two infrared photons simultaneously, it will absorb enough energy to be raised into the excited state.
45.
This derivitization with a fluorophore permits both separation on a gel under the desired circumstances and fluorescence imaging of the gel.
46.
Blood plasma sample is periodically irradiated with the excitation light and the emission of the fluorophore is registered by CCD camera.
47.
It can be conjugated to a fluorophore or enzyme for immunohistochemical staining of fixed tissues and visualization via light or fluorescence microscopy.
48.
If only the first probe s fluorophore wavelength is detected during the assay then the individual is homozygous to the wild type.
49.
A common problem in fluorescence microscopy is photobleaching, where the fluorophore loses its activity as a result of exposure to light.
50.
However, any obvious advantages are outweighed by three-color system s requirements which includes a clear separation of fluorophore signals.
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