41. A 2014 study of NGLY1 deficient patients found similarities with traditional congenital disorders of glycosylation . 42. Data suggest three separate glycosyl transferases are required for the glycosylation of the teicoplanin aglycone. 43. The O-linked glycosylation is necessary to target the protein to the apical membrane. 44. The additional molecular weight is partly due to a single glycosylation site at residue 285. 45. There are 3 consensus sequences for N-glycosylation and 1 chondroitin sulfate substitution site. 46. Glycosylation of hydroxylysine starts in the ER, but occurs predominantly in the Golgi apparatus.47. E1 has 4 5 N-linked glycans and E2 has 11 N-glycosylation sites. 48. The same study also identified 18 rare alleles, which generally have a weaker glycosylation activity. 49. Processing of proBNP may be regulated by O-glycosylation of residues near the cleavage sites. 50. The specificity of TAP proteins was first investigated by trapping peptides in the ER using glycosylation .