On day five post-fertilization, approximately five cells are excised from the TE using a glass needle or laser energy, leaving the embryo largely intact and without loss of inner cell mass.
42.
On the deep surface of the inner cell mass, a layer of flattened cells, called the endoderm, is differentiated and quickly assumes the form of a small sac, called the yolk sac.
43.
The cells develop into pluripotent cells ( the inner cell mass ) where the imprint is removed, which leads to the downregulation of Xist and thus reactivation of the inactive X chromosome.
44.
On day 4, embryos were scored in function of their degree of compaction and blastocysts were evaluated according to the quality of the throphectoderm and inner cell mass, and their degree of expansion.
45.
The blastocyst contains an embryoblast ( or inner cell mass ) that will eventually give rise to the definitive structures of the fetus, and the trophoblast, which goes on to form the extra-embryonic tissues.
46.
After an egg is fertilized it divides several times and forms a blastocyst, a hollow sphere with a blob of 15 to 20 cells, known as the inner cell mass, piled up against one wall.
47.
The inner cell mass remains in contact, however, with the trophoblast at one pole of the ovum; this is named the embryonic pole, since it indicates the location where the future embryo will be developed.
48.
As the number of cells grows from 16 to between 40 and 150, the blastocyst differentiates into two layers, an outer sphere of cells called the trophoblast and an inner cell mass called the embryoblast.
49.
The floor of this cavity is formed by the embryonic disk, which is composed of a layer of prismatic cells, the embryonic ectoderm, derived from the inner cell mass and lying in apposition with the endoderm.
50.
Allan Robins, BresaGen's chief scientific officer, said his firm has focused on isolating cells from a stage of embryonic development called the early primitive ectoderm _ which occurs slightly after the formation of the inner cell mass.
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