41. The 10.5kDa protein SH3 binding glutamic acid-rich protein-like 3 has an isoelectric point of 5.0. 42. Separation of proteins may be by isoelectric point ( pI ), molecular weight, electric charge, or a combination of these factors. 43. First, proteins may be purified according to their isoelectric points by running them through a pH graded gel or an ion exchange column. 44. The bovine protein is homologous with other mammalian species and is a homogeneous 50 kDa glycoprotein with an isoelectric point of 6.5. 45. It has a predicted molecular weight of 33.6 kdal and an isoelectric point of 6.496000, making it slightly acidic. 46. The structure of KIAA0452 is predicted to have 11 alpha helices and 9 beta sheets . and an isoelectric point of 6.766000. 47. The human protein has a molecular weight of 17.6 kiloDaltons ( kD ), and an isoelectric point of 5.56. 48. Thus, point of zero charge at the surface is taken as equal to isoelectric point in the absence of specific adsorption on that surface. 49. Although most yeasts have only a single actin gene, higher eukaryotes, in general, beta and gamma ) according to their isoelectric points . 50. The isoelectric point of the protein is 4.21, the acidity of which may be attributed to the abundance of these amino acids.
