Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin ( 50-100 ug / ml ) containing agar plates or are grown in media containing kanamycin ( 50-100 ug / ml ).
42.
Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin ( 50-100 ug / ml ) containing agar plates or are grown in media containing kanamycin ( 50-100 ug / ml ).
43.
Bacteria that have been transformed with a plasmid containing the kanamycin resistance gene are plated on kanamycin ( 50-100 ug / ml ) containing agar plates or are grown in media containing kanamycin ( 50-100 ug / ml ).
44.
EHA101 is resistant to kanamycin by way of an npt I gene in place of T-DNA . The parent strain, A281, does not show antibiotic resistances at higher levels than normal " A . tumefaciens " strains.
45.
Some examples of these compounds that contain these mixtures are as follows : Azithromycin, Bacitracin, Bleomycin, Clarithromycin, Cyclosporin, Enramycin, Erythromycin, G418, Gentamicin, Hygromycin, Kanamycin, Neomycin, Polymyxin, Roxithromycin, Tetracycline, Tylosin.
46.
At this point the kanamycin pathway splits into two branches due to the promiscuity of the next enzyme, which can utilize two different glycosyl donors-UDP-N-acetyl-?-D-glucosamine and UDP-?-D-glucose.
47.
One of the branches forms kanamycin C and kanamycin B, while the other branch forms kanamycin D and kanamycin X . However, both kanamycin B and kanamycin D can be converted to kanamycin A, so both branches of the pathway converge at kanamycin A.
48.
One of the branches forms kanamycin C and kanamycin B, while the other branch forms kanamycin D and kanamycin X . However, both kanamycin B and kanamycin D can be converted to kanamycin A, so both branches of the pathway converge at kanamycin A.
49.
One of the branches forms kanamycin C and kanamycin B, while the other branch forms kanamycin D and kanamycin X . However, both kanamycin B and kanamycin D can be converted to kanamycin A, so both branches of the pathway converge at kanamycin A.
50.
One of the branches forms kanamycin C and kanamycin B, while the other branch forms kanamycin D and kanamycin X . However, both kanamycin B and kanamycin D can be converted to kanamycin A, so both branches of the pathway converge at kanamycin A.
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