Traditionally DNA microarrays use complementary DNA or oligonucleotide probes to analyze messenger RNA ( mRNA ) from genes of interest.
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The best antisense oligonucleotide was identified that effectively down-regulated survivin mRNA levels and resulted in apoptosis of the cells.
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Eurogentec operates two manufacturing facilities in Belgium that provide custom biologics and oligonucleotide-based components for diagnostic and therapeutic applications.
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DNA sequencing methods improved greatly ( pioneered by Frederick Sanger and Walter Gilbert ), as did oligonucleotide synthesis and transfection techniques.
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Often, an extra lane is run with a competitor oligonucleotide to determine the most favorable binding sequence for the binding protein.
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Oligonucleotide phosphorothioates ( OPS ) are modified oligonucleotides where one of the oxygen atoms in the phosphate moiety is replaced by sulfur.
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Massively parallel sequencing has also been used as a tool to screen complex oligonucleotide libraries and enable the retrieval of accurate molecules.
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The oligonucleotide probe hybridizes with the repeat in the microsatellite, and the probe / microsatellite complex is then pulled out of solution.
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Antisense oligonucleotide ( ASO ) technology can be used to silence the expression of the NNMT gene only in fat and liver cells.
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DNA sequences are known to appear in the form of synthetic oligonucleotide strands to hybridize in such a way as to perform computation.
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