Uses fluorescent resonance energy transfer ( FRET ) to enhance its automated RT-PCR . In the FRET reaction, a donor and acceptor probe exchange excitation energy when within 1-5 base pairs ( bp ) on the target sequences.
42.
To obtain depth information the initial kinetic energy of the projectile nucleus ( which has to exceed the resonance energy ) and its stopping power ( energy loss per distance traveled ) in the sample has to be known.
43.
The energy of the exciton is transferred to a chlorophyll molecule ( P680, where P stands for pigment and 680 for its absorption maximum at 680 nm ) in the reaction center of photosystem II via resonance energy transfer.
44.
Experimental methods for the determination of binding affinities are : surface plasmon resonance ( SPR ), F�rster resonance energy transfer, radioligand-based techniques, isothermal titration calorimetry ( ITC ), microscale thermophoresis ( MST ) or spectroscopic measurements and other fluorescence techniques.
45.
Since photobleaching consists in the permanent inactivation of excited fluorophores, resonance energy transfer from an excited donor to an acceptor fluorophore prevents the photobleaching of that donor fluorophore, and thus high FRET efficiency leads to a longer photobleaching decay time constant:
46.
He completed his PhD at Stanford in 1970 under Lubert Stryer, showing in a now widely cited and classic paper that F�rster resonance energy transfer ( FRET ) can be used as a " spectroscopic ruler " to measure distances in macromolecules.
47.
Recently the Shaker potassium ion channel, Some scientist also have used lanthanide based luminescence resonance energy transfer ( LRET ) which is very similar to FRET to study conformational changes in RNA polymerase upon binding to DNA and transcription initiation in prokaryotes.
48.
However, due to the closely associated GFP, the energy released by the luciferase is instead coupled through resonance energy transfer to the fluorophore of the GFP, and is subsequently released as a photon of green light ( peak emission wavelength 510 nm ).
49.
His research at Stanford was a combination of organic synthesis of novel fluorescent dyes and experimental proofs of the theory of fluorescence resonance energy transfer ( FRET ), a physical effect that permits measurement of distances in the range of the size of proteins.
50.
This Fluorescence Resonance Energy Transfer ( FRET ), as oligoribonucleotides containing a quencher and a fluorophore on opposite sites are added to a solution with inactive RNase L . The FRET signal is then recorded as the quencher and the fluorophore are very close to each other.
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