41. Further complications could arise when incomplete digestion of DNA by restriction enzymes generated false negative results. 42. These standard sequences encode specific restriction enzyme sites. 43. Nathan found that the restriction enzyme cut the simian virus in 10 places, creating 11 well-defined fragments. 44. It involves the use of restriction enzymes . 45. Use of restriction enzymes or PCR amplification on the DNA is required for RFLP or RAPD analysis respectively. 46. In a similar manner, restriction enzymes are used to digest genomic DNA for gene analysis by Southern blot. 47. Restriction enzymes commonly used in molecular biology to cut DNA interact with sequences of 1 to 10 nucleotides.48. Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. 49. These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. 50. Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ).
