41. DNA is digested with the restriction enzyme AluI, many 1109 base-pair fragments were obtained. 42. PCR products are then treated with a restriction enzyme ( " e . g ." 43. Restriction enzymes commonly used in molecular biology to cut DNA interact with sequences of 1 to 10 nucleotides.44. Use of restriction enzymes or PCR amplification on the DNA is required for RFLP or RAPD analysis respectively. 45. Over 3000 restriction enzymes have been studied in detail, and more than 600 of these are available commercially. 46. In a similar manner, restriction enzymes are used to digest genomic DNA for gene analysis by Southern blot. 47. These enzymes became known as restriction enzymes and developed into one of the main molecular tools in molecular biology. 48. Restriction endonucleases or restriction enzymes typically cleave in two ways : blunt-ended or sticky-ended patterns. 49. Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ). 50. Most commonly used restriction enzymes are Type II restriction endonuclease ( See article on Restriction enzymes for examples ).
