41. Glutaminyl-tRNA synthetase belongs to the class-I aminoacyl-tRNA synthetase family. 42. The active site of the synthetase is modified to accept only the novel amino acid. 43. It is formed by glutamate cysteine ligase and used by glutathione synthetase to form glutathione. 44. L-Canavaninosuccinic acid is cleaved to form L-canavanine by argininosuccinic acid synthetase . 45. The enzyme aminoacyl tRNA synthetase " charges " the tRNA molecules with the correct amino acids. 46. Thus, glutamate tRNA synthetase charges tRNA-glutamine ( tRNA-Gln ) with glutamate. 47. All of these reactions take place inside the aminoacyl-tRNA synthetase specific for that tRNA. 48. In one family mutations in the mitochondrial histidyl tRNA synthetase have been described as the cause. 49. This enzyme is also called "'nondiscriminating asparaginyl-tRNA synthetase " '. 50. Indeed, SIRT3 deacetylates and activates the mammalian mitochondrial acetyl-coA synthetase ( AceCS2 ).
