1. Aldolase A deficiency has been associated with myopathy and hemolytic anemia.2. In bacterial systems, sialic acids are biosynthesized by an aldolase enzyme. 3. Aldolase B converts it into glyceraldehyde and dihydroxyacetone phosphate ( DHAP ).4. This cleavage is very similar mechanistically to the aldolase A reaction of glycolysis. 5. In lymphocytes, ALDOA is the predominant aldolase isoform. 6. The active site of this archaeal aldolase is also in a TIM barrel. 7. Aldolase is divided into two classes by mechanism.8. Each subunit contains a phosphate-ion bound in position of the aldolase biding site. 9. Genetic mutations leading to defects in aldolase B result in a condition called hereditary fructose intolerance. 10. This is the same mechanism proposed by Barbas for aldolase antibodies reported by the group in 1995:
