1. Denatonium benzoate is the most widely accepted ethanol denaturant worldwide. 2. Protein stabilities are typically found to vary linearly with the denaturant concentration. 3. When used to characterize protein folding, it measures the denaturant or temperature. 4. Folding intermediates for this protein can be studied using light spectroscopy and denaturant . 5. One thus obtains a profile relating the global protein signal to the denaturant value. 6. Denaturants 6M-urea or 4M-guanidine hydrochloride did not destroy the protein.7. There are several grades of denatured alcohol, but in general the denaturants used are similar. 8. As in the denaturant -binding model, fitting to this model also requires 7 parameters. 9. Brucine is one of the many chemicals used as a denaturant to make alcohol unfit for human consumption. 10. The resulting slope of the plot of stability versus the denaturant concentration is called the m-value.
