1. One of the cell cycle checkpoints occurs during prometaphase and metaphase. 2. Both Aurora B and MCAK localize to the inner centromere during prometaphase . 3. These stages are prophase, prometaphase , metaphase, anaphase, and telophase. 4. Scheme showing cell cycle progression between prometaphase and anaphase. 5. Aurora A also assures proper organization and alignment of the chromosomes during prometaphase . 6. DYNLT3 binds to BUB3, a spindle checkpoint protein is present on kinetochores at prometaphase . 7. During prometaphase , CDC20 and the SAC proteins concentrate at the kinetochores before attachment to the spindle assembly. 8. In late prometaphase , " kinetochore microtubules " begin to search for and attach to chromosomal kinetochores. 9. The preprophase band also functions in properly orienting the mitotic spindle, and contributes to efficient spindle formation during prometaphase 10. As one of the checkpoint proteins, Bub3 delays the irreversible onset of anaphase through direction of kinetochore localization during prometaphase
