electrophoretic mobility sentence in Hindi
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- Thus, the electrophoretic mobility depends not only on the charge-to-mass ratio, but also on the physical shape and size of the protein.
- Charged analytes have a more complex interaction in the capillary because they exhibit electrophoretic mobility, engage in electrostatic interactions with the micelle, and participate in hydrophobic partitioning.
- Historically, the experimental techniques of choice to discover and analyze DNA binding sites have been the DNAse footprinting assay and the Electrophoretic Mobility Shift Assay ( EMSA ).
- The approach of MLST is distinct from Multi locus enzyme electrophoresis ( MLEE ), which is based on different electrophoretic mobilities ( EM ) of multiple core metabolic enzymes.
- Other biological macromolecules that can be analyzed with ELS include polysaccharides . pKa values of chitosans can be calculated from the dependency of electrophoretic mobility values on pH and charge density.
- Denaturing conditions are necessary for proper estimation of molecular weight of RNA . RNA is able to form more intramolecular interactions than DNA which may result in change of its electrophoretic mobility.
- It has been used in the electrophoretic mobility shift assay and p50 and title comp preincubated in assay buffer, pH 7.5 ( room temp ., 5 min ).
- Through combination with fine-control of pressure TRPS has been used to determine sample concentration and to accurately derive particle electrophoretic mobility & surface charge in addition to particle size information.
- Since the electroosmotic flow of the buffer solution is generally greater than that of the electrophoretic mobility of the analytes, all analytes are carried along with the buffer solution toward the cathode.
- It uses no charged dye so the electrophoretic mobility of proteins in CN-PAGE ( in contrast to the charge shift technique BN-PAGE ) is related to the intrinsic charge of the proteins.
- Since velocity \ V \, is proportional to the applied electric field, \ E \,, the apparent electrophoretic mobility \ \ mu _ { obs } \, is define by the equation
- Assays are created that combine reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence microscopy to silence the T6SS gene cluster by the histone-like nucleoid structuring H-NS protein.
- Proteins which associate with the promoter can be identified using an electrophoretic mobility shift assay ( EMSA ), and the effects of inclusion or exclusion of the proteins with the mutagenized promoters can be assessed in the assay.
- He found that, under certain conditions, sickle cell hemoglobin is positively charged while normal hemoglobin is not, creating a difference in electrophoretic mobility . which by 1958 he determined to be a glutamic acid in place of a valine.
- Since electrophoretic mobility of ions is generally temperature-dependent ( due to both temperature-dependent ionization and solvent viscosity effects ), a non-uniform temperature profile results in variation of electrophoretic mobility across the capillary, and a loss of resolution.
- Since electrophoretic mobility of ions is generally temperature-dependent ( due to both temperature-dependent ionization and solvent viscosity effects ), a non-uniform temperature profile results in variation of electrophoretic mobility across the capillary, and a loss of resolution.
- In some very specific cases, the detection of the phosphorylation as a shift in the protein's electrophoretic mobility is possible on simple 1-dimensional SDS-PAGE gels, as it's described for instance for a transcriptional coactivator by Kovacs et al.
- They introduced a new terminology for this product, calling it " Form V " ( extending the nomenclature of Forms I IV used in sedimentation studies of DNA ), and then went on to demonstrate that its electrophoretic mobility was clearly different from that of the native chromosome.
- FANCA binds to both single-stranded ( ssDNA ) and double-stranded ( dsDNA ) DNAs; however, when tested in an electrophoretic mobility shift assay, its affinity for ssDNA is significantly higher than for dsDNA . FANCA also binds to RNA with a higher affinity than its DNA counterpart.
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