expression vector sentence in Hindi
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- Simpler vectors called transcription vectors are only capable of being transcribed but not translated : they can be replicated in a target cell but not expressed, unlike expression vectors.
- "' Expression cloning "'is a technique in DNA cloning that uses expression vectors to generate a library of clones, with each clone expressing one protein.
- The fully sequenced genome of " M . tuberculosis " has allowed scientists to clone many of these protein targets into expression vectors for purification and structure determination by X-ray crystallography.
- For example, if the experimentalists wish to harvest a particular protein from the recombinant organism, then an expression vector is chosen that contains appropriate signals for transcription and translation in the desired host organism.
- In a popular expression vector, the desired protein is produced as a fusion product to the secretion signal of the ?-mating factor from " Saccharomyces cerevisiae " ( baker's yeast ).
- The software is a bioinformatics tool developed and maintained by GenoFAB . GenoCAD facilitates the design of protein expression vectors, artificial gene networks and other genetic constructs for genetic engineering and is based on the theory of formal languages.
- The inducible, meaning that protein synthesis is only initiated when required by the introduction of an inducer such as IPTG . Gene expression however may also be constitutive ( i . e . protein is constantly expressed ) in some expression vectors.
- These recombinant proteins have been used in research and as vaccines in both human and veterinary medical treatments ( for example, the most widely used vaccine for prevention of H5N1 avian influenza in chickens was produced in a baculovirus expression vector ).
- Most expression vectors for extrachromosomal protein expression and shuttle vectors contain the pBR322 origin of replication, and fragments of pBR322 are very popular in the construction of intraspecies shuttle or binary vectors and vectors for targeted integration and excision of DNA from chromosome.
- Cloning vectors without promoter and RBS for the cloned DNA sequence are sometimes used, for example when cloning genes whose products are toxic to " genomic or cDNA library of clones since the cloned genes are normally subcloned into a more appropriate expression vector if their expression is required.
- Examples of " E . coli " expression vectors are the pGEX series of vectors where glutathione S-transferase is used as a fusion partner and gene expression is under the control of the tac promoter, and the pET series of vectors which uses a T7 promoter.
- The Sh " ble " gene product binds the antibiotic in a one-to-one ratio so it can no longer cause cleavage of DNA . This resistance gene is used as a selectable marker in some cloning and expression vectors where zeocin is used as the antibiotic for selection.
- "Nde " I is useful in generating heterologous DNA construct because it contains the start codon ATG . It therefore can be in used in some expression vectors, such as those in the pET series of vectors, for the ligation of the start of a gene when making an expression construct.
- An expression vector has features that any Escherichia coli ", and vectors used for protein production in organisms other than " E . coli " may have, in addition to a suitable origin of replication for its propagation in " E . coli ", elements that allow them to be maintained in another organism, and these vectors are called shuttle vectors.
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