microinjection sentence in Hindi
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- PPL said in a statement, " techniques such as microinjection allowed only the introduction of new genetic material into an animal ."
- Due to the damage caused to the cells and DNA the transformation efficiency of biolistics and electroporation is lower than agrobacterial mediated transformation and microinjection.
- Live cell imaging of RNA can be achieved by introducing synthesized RNA that is chemically coupled with a fluorescent tag into living cells by microinjection.
- Inhibition of Aurora B function by RNA interference or microinjection of blocking antibodies impairs the alignment of chromosomes at the equator of the mitotic spindle.
- Microinjection of CdtB into susceptible cells without CdtA or CdtC results in the G2 / M cell cycle arrest and cytoplasmic distension characteristic of CDT toxins.
- Once an animal is produced, typically using the pronuclear microinjection method, it becomes efficacious to use cloning technology to create additional offspring that carry the favorable modified genome.
- In the context of existing approaches to gene transfer, PAMAM dendrimers hold a strong position relative to major classical technologies such as electroporation, microinjection, and viral methods.
- He was the first scientist to microinject fertilized eggs with RNA and DNA, and was at the forefront of the field in applying these microinjection methods to generate transgenic mice.
- This construct was introduced into a fertilized embryo by pronuclear microinjection, and this embryo along with other embryos was surgically implanted into the reproductive tract of an estrous synchronized sow.
- Microinjection of glutamic acid into neurons produces spontaneous depolarisations around one second apart, and this firing pattern is similar to what is known as paroxysmal depolarizing shift in epileptic attacks.
- Non-viral methods include physical methods such as electroporation, microinjection, gene gun, impalefection, hydrostatic pressure, continuous infusion, and sonication and chemical, such as lipofection.
- Prior to the production of Polly and Molly, the only demonstrated way to make a transgenic animal was by microinjection of DNA into the pronuclei of fertilized oocytes ( eggs ).
- To initiate parthenogenesis of swine oocytes, various methods exist to induce an artificial activation that mimics sperm entry, such as calcium ionophore treatment, microinjection of calcium ions, or electrical stimulation.
- Microinjection can also be used in the cloning of organisms, in the study of cell biology and viruses, and for treating male subfertility through intracytoplasmic sperm injection ( ICSI, " " ).
- For this purpose, specific cells were ablated by microinjection with Pronase ( an enzyme that digests proteins ) to ablate the cell; later modifications of this technique employed DNase or the ricin A chain.
- In contrast to the study above, another group of researchers found that microinjection of SP into the RVM resulted in transient thermal hyperalgesia, which persisted long-term when continuous infusion pumps were implanted.
- The construct can be inserted in the plant genome by genetic recombination using the bacteria " Agrobacterium tumefaciens " or " A . rhizogenes ", or by direct methods like the gene gun or microinjection.
- Furthermore, it is important to note that phalloidin-treated cells will have greater levels of actin associated with their plasma membranes, and the microinjection of phalloidin into living cells will change actin distribution as well as cell motility.
- As the aim of such research is to produce an animal that expresses a particular protein in high levels in, for example, its milk, microinjection is a very costly procedure that does not usually produce the desired animal.
- Cells with impaired function of Ndc80 ( using RNAi, gene knockout, or antibody microinjection ) have abnormally long spindles, lack of tension between sister kinetochores, chromosomes unable to congregate at the metaphase plate and few or any associated kMTs.
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