Our previous studies showed existence of apoplast cam in the plant cell and cam had many extracellular functions . so it supposed cam may be one of important extracellular polypeptides and trigger the intracellular signal transduction by binding the receptor . in this study , radiolabelled ligand is used to investigate the binding characteristic of cam and a . thaliana protoplasts . and chemical crosslinking is employed to explore binding proteins in the membrane . at first , ( 35 ) ~ s - cam was produced using ( 35 ) ~ s - labeled amino acid mixture in e . coli . . sds - page and autoradiograph indicated high - purified , high - specific radioactivity ( 35 ) ~ s - cam was obtained . electrophoresis of ( 35 ) ~ s - cam is the same as that of unlabeled cam with ca ~ ( 2 + ) or egta ; a quatitive of protoplasts was prepared by enzymolysis
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