Sucrose gradients are typically used for separation of cellular organelles.
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However, when the proteins are moving through a sucrose gradient, they encounter liquid of increasing density and viscosity.
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A properly designed sucrose gradient will counteract the increasing centrifugal force so the particles move in close proportion to the time they have been in the centrifugal field.
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Why doesn't the sucrose in a sucrose gradient diffuse to reach equilibrium and thereby eliminate the gradient ? Preceding talk ) 15 : 32, 14 May 2013 ( UTC)
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Sucrose gradient centrifugation & mdash; a linear concentration gradient of sugar ( typically sucrose, glycerol, or a silica based density gradient media, like Percoll ) is generated in a tube such that the highest concentration is on the bottom and lowest on top.
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