1. Acridine orange is useful for enumerating the microbes in a sample.2. Like the related molecule pyridine and quinoline, acridine is mildly basic. 3. It is a precursor to certain acridine dyes as well. 4. There are no commercial applications of acridines but at one time acridine dyes were popular. 5. Examples include ethidium bromide and acridine . 6. Acridine orange direct count ( AODC ) methodology has been used in the enumeration of landfill bacteria.7. Condensation of 2, 4-diaminotoluene with acetaldehyde gives the acridine dye called Basic Yellow 9. 8. Acridine orange binding with the nucleic acid occurs in both living and dead bacteria, also other microorganisms.9. The use of polyphosphoric acid will give acridine products at a lower temperature, but also with decreased yields. 10. Acridine orange can be used in conjunction with ethidium bromide to differentiate between viable, apoptotic and necrotic cells.
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