The enzymes used in the saccharification step are the amylolytic enzyme, beta-amylase ( usually derived from " Bacillus species " ) and the debranching enzyme, pullulanase ( derived from " Aerobacter species " ).
2.
"E . cloacae " was described for the first time in 1890 by Jordan [ 201 ] as " Bacillus cloacae ", and then underwent numerous taxonomical changes, becoming'Bacterium cloacae'in 1896 ( Lehmann and Neumann ), " Cloaca cloacae " in 1919 ( Castellani and Chalmers ), it was identified as'Aerobacter cloacae'in 1923 ( Bergey et al . ), " Aerobacter cloacae " in 1958 ( Hormaeche and Edwards ) and " E . cloacae " in 1960 ( Hormaeche and Edwards ), by which it is still known today . [ 7 ] " E . cloacae " is ubiquitous in terrestrial and aquatic environments ( water, sewage, soil and food ).
3.
"E . cloacae " was described for the first time in 1890 by Jordan [ 201 ] as " Bacillus cloacae ", and then underwent numerous taxonomical changes, becoming'Bacterium cloacae'in 1896 ( Lehmann and Neumann ), " Cloaca cloacae " in 1919 ( Castellani and Chalmers ), it was identified as'Aerobacter cloacae'in 1923 ( Bergey et al . ), " Aerobacter cloacae " in 1958 ( Hormaeche and Edwards ) and " E . cloacae " in 1960 ( Hormaeche and Edwards ), by which it is still known today . [ 7 ] " E . cloacae " is ubiquitous in terrestrial and aquatic environments ( water, sewage, soil and food ).
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