1. For some purposes, certain bacteria are able to hydrolyze aesculin . 2. A plate containing aesculin will fluoresce a pale blue under UV radiation. 3. Aesculin , the glucoside of aesculetin, will nm ).4. The hydrolysis of aesculin results in loss of this fluorescence. 5. The bile aesculin agar is streaked and incubated at 37 �C for 24 hours. 6. Aesculin will nm ) : hydrolysis of aesculin results in loss of this fluorescence.7. Aesculin will nm ) : hydrolysis of aesculin results in loss of this fluorescence. 8. This species is able to hydrolyse aesculin . 9. It hydrolyses casein and gelatin but not Tween 80, starch, lecithin, aesculin or tyrosine. 10. The USDA notes that the toxicity is due to saponin aescin and glucoside aesculin , with alkaloids possibly contributing.