1. There are two categories of decalcifying agents namely, chelating agents and acids. 2. Deferoxamine, deferiprone and deferasirox are the three most widely used iron-chelating agents . 3. This mixture contains ammonium thiosulphate and ferrous EDTA, a powerful chelating agent . 4. Protein samples usually contains salts, solvents, buffers, preservatives, and metal chelating agents . 5. Toxic metal poisoning is usually treated with some type of chelating agent . 6. Treatment with chelating agents such as EDTA leads to complete inactivation. 7. Most frequently used chelating agent is Ethylenediaminetetraacetic acid ( EDTA ). 8. It is used widely as an acidifier, as a flavoring and chelating agent . 9. Other approaches include precipitation methods and sequestration by the addition of chelating agents . 10. Exposure can cause iron poisoning, which can be treated with deferoxamine, a chelating agent .